師資
個人簡介
劉東實驗室現(xiàn)有的兩個研究主題是：1）比較器官發(fā)育與再生的異同；2）建立人類疾病的動物模型。劉東博士長期從事硬骨魚類生物學(xué)研究，涉足發(fā)育、分子遺傳學(xué)（斑馬魚和日本青鳉），分子內(nèi)分泌學(xué)（太平洋鮭鱒），轉(zhuǎn)基因（鯉科魚）、基因編輯技術(shù)（斑馬魚）等領(lǐng)域。我們主要是用模式動物斑馬魚研究動感毛細(xì)胞（mechanosensory hair cells）發(fā)育及其損傷-再生機(jī)制，并在建立神經(jīng)性疾病模型基礎(chǔ)上研發(fā)治療性或預(yù)防性藥物。此外，運(yùn)用線蟲的優(yōu)勢，我們還在探索研究器官發(fā)育與再生的新思路、新方法。
研究領(lǐng)域
1. 斑馬魚內(nèi)耳（外周神經(jīng)系統(tǒng)器官）的發(fā)育機(jī)制
2. 斑馬魚側(cè)線毛細(xì)胞損傷/再生機(jī)制
3. 構(gòu)建人類神經(jīng)性疾病的斑馬魚模型，并進(jìn)行治療性先導(dǎo)化合物的篩選，靶標(biāo)確定及作用機(jī)理研究
4. 基因組編輯技術(shù)研發(fā)
具體研究內(nèi)容
1. 斑馬魚內(nèi)耳的發(fā)育：通過遺傳學(xué)和條件性調(diào)控基因表達(dá)等手段，已初步揭開從前基板區(qū)（一種在體干細(xì)胞群）到內(nèi)耳細(xì)胞命運(yùn)獲得的發(fā)育途徑,詮釋內(nèi)耳功能細(xì)胞(內(nèi)耳神經(jīng)元和動感受體毛細(xì)胞)命運(yùn)決定和維持的調(diào)控網(wǎng)絡(luò)
2. 斑馬魚側(cè)線毛細(xì)胞損傷/再生：位于斑馬魚體表的側(cè)線神經(jīng)丘是毛細(xì)胞損傷/再生研究的好模型。通過突變體篩選，活體細(xì)胞分選和基因表達(dá)調(diào)控技術(shù)，已初步確定信號通路，轉(zhuǎn)錄因子和死亡程序與毛細(xì)胞再生現(xiàn)象間的關(guān)聯(lián)性。
3. 人類疾病的斑馬魚模型：已建立耳聾，多動癥和帕金森模型，并初選治療性先導(dǎo)化合物。
4. 新技術(shù)、新方向：已開發(fā)出鑒定有功能非蛋白編碼RNA，CRISPR/Cas9 基因編輯，dCas9 融合系統(tǒng)，亞細(xì)胞定位等技術(shù)；生殖細(xì)胞與體細(xì)胞性別發(fā)育的關(guān)聯(lián)研究。
工作經(jīng)歷
2017-至今 南方科技大學(xué)生物系，副教授
2008-2017 北京大學(xué)生命科學(xué)學(xué)院，研究員, 博士生導(dǎo)師
1999-2008 俄勒岡大學(xué)神經(jīng)所，博士后轉(zhuǎn)高級助研（senior research associate）
1990-1991 淡水生態(tài)學(xué)及生物技術(shù)國家重點(diǎn)實驗室（中科院武漢水生所），助研（research assistant）
教育背景
1999-2004 俄勒岡大學(xué)神經(jīng)所，發(fā)育生物學(xué)博士后
1992-1999 多倫多大學(xué)生化系，分子內(nèi)分泌學(xué)碩士、博士（PhD，哲學(xué)博士）
1987-1990 中國科學(xué)院水生生物研究所，遺傳學(xué)碩士
1983-1987 武漢大學(xué)生物系，理學(xué)學(xué)士
獲獎情況及榮譽(yù)
1. Travel Award, International Zebrafish Society, 2017（國際斑馬魚協(xié)會）
2. Roche Fellowship Award, School of Life Sciences, PKU 2012 （羅氏獎教金）
3. Peking University Talents Program, 2008-2011 (北京大學(xué)引進(jìn)人才)
4. Hundred Talent Program nominee, Institute of Hydrobiology, the Chinese Academy of Sciences, 2008 (declined)
5. Hundred Talent Program nominee, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, 2004(declined)
6. Travel Award, Society of Chinese Bioscientists in America, 2004 （美洲華人生物科學(xué)者協(xié)會）
7. Travel award, Association for Research in Otolaryngology, 2003 （美國耳鼻喉科研究協(xié)會）
8. Postdoctoral Fellowship, Canadian Institutes of Health Research, 2000 -2003 （加拿大健康研究院）
9. JSPS Postdoctoral Fellowship, 1999-2001（日本學(xué)術(shù)振興學(xué)會）(declined)
10. Restracom Trainee Fellowship, HSC, Toronto, 1994 -1999 （加拿大病童醫(yī)院研究所）
11. University of Toronto Open Fellowship, 1991-1992（多倫多大學(xué)）
獲資助情況
1. Principal Investigator, Establishing a new model of mechanosensory hair cell damage/regeneration (斑馬魚動感毛細(xì)胞損傷/再生新模型的建立), a NSFC regular grant (2018-2021).
2. Principal Investigator, Dlx3b-4b and Gata3 regulate otic hair cell development in zebrafish (Dlx3b-4b和Gata3是斑馬魚內(nèi)耳毛細(xì)胞發(fā)育的主要調(diào)節(jié)因子), a NSFC regular grant (2016-2019).
3. Principal Investigator, Systematic characterization and analysis of tissue/cell-specific lincRNAs in elegans (系統(tǒng)性鑒定和分析線蟲組織/細(xì)胞特異（基因間區(qū)長片段非編碼RNA）, a NSFC regular grant (2014-2017).
4. Principal Investigator, Developing new techniques for organ regeneration study, part of a MOST 973 Program (2012-2016). （第三課題組長）
5. Co-Principal Investigator, In vivo functional study of lincRNAs, part of a MOST 973 Program (2011-2015). （骨干PI）
6.Principal Investigator, Epigenetic regulation of sex differentiation and reproductive development(性別分化及生殖發(fā)育的表觀遺傳調(diào)控研究), a NSFC regular grant (2010-2012, ‘細(xì)胞編程與重編程的表觀遺傳機(jī)制’重大研究計劃).
NSFC：國家自然科學(xué)基金委；MOST：中華人民共和國科學(xué)技術(shù)部
代表文章（*：通訊或共同通訊作者）
1. Li J, Liu Y, Qin L, Huang X, Zhou D, Xu H, Zhao F, Mi X, Jia F, Xu F, Yang J, Li C, Liu D, Deng X, Zhang Y. (2017). Mutation in ε-sarcoglycan induces myoclonus dystonia syndrome by increasing the numbers of excitatory synapses. Submitted.
2. Wu YT, Mi X, Guo H, Zhao F, Zhang Y, Tang G, Yu K, Zhao J, Su X, Zhu Z, Raible DW, Liu D*. (2017). Regenerating neuromast hair cells directly from proliferative supporting cells in zebrafish. Revised
3. Li X, Song G, Zhao Y, Zhao F, Liu C, Liu D*, Li Q*, Cui Z*. (2017). Claudin7b is required for the formation and function of inner ear in zebrafish. J Cell Physiol (doi: 10.1002/jcp.26162. [Epub ahead of print]).
4. Yang L, Chang S, Lu Q, Zhang Y, Wu Z, Sun X, Cao Q, Qian Y, Jia T, Xu B, Duan Q, Li Y., Zhang K, Schumann G, Liu D*, Wang J*, Wang Y*, Lu L.（2017）. A new locus regulating MICALL2 expression was identified for association with executive inhibition in children with attention deficit hyperactivity disorder. Mol Psychiatry(doi:10.1038/mp.2017.74).
5. Gao H, Bu Y, Wu Q, Wang X, Chang N, Lei L, Chen S, Liu D, Zhu X, Hu K, Xiong J. (2015). Mecp2 regulates neural cell differentiation by suppressing the Id1-Her2/Hes5 axis in zebrafish. J Cell Sci, 128(12):2340-2350.
6. Long L, Guo H, Yao D, Xiong K, Li Y, Liu P*, Zhu Z, Liu D*. (2015). Regulation of transcriptionally active genes via the catalytically inactive Cas9 in elegans and D. rerio. Cell Res, 25:638-641.
7. Wang J, Zhao F, Wu Y, Wu YT, Yao D, Dong W, Zhao J, Zhu Z, Liu D*. (2015). Fgf signaling dependent Sox9a and Atoh1a regulate otic neural development in zebrafish. J Neuroscience, 35:234-244 (highlighted article).
8. Yao D, Zhao F, Wu Y, Wang J, Zhao J, Zhu Z, Liu D*. (2014). Dissecting the differentiation process of the pre-placodal ectoderm in zebrafish. Special issue ‘Sensory system: Development, Disease & Regeneration’, Dev Dyn, 243:1328-1337.
9. Liu P, Long L, Xiong K, Yu B, Chang N, Xiong J-W, Zhu Z, Liu D*. (2014). Heritable/Conditional Genome Editing in elegans using a CRISPR-Cas9 Feeding System. Cell Res, 24:886-889.
10. Sheehan-Rooney K, Swartz ME, Zhao F, Liu D, Eberhart JK. (2013). Ahsa1 and Hsp90 activity confers more severe craniofacial phenotypes in a zebrafish model of hypoparathyroidism, sensorineural deafness and renal dysplasia (HDR). Dis Model Mech, 6(5):1285-91.
11. Dong W, Yang Z, Yang F, Wang J, Zhuang Y, Xu C, Zhang B, Tian X*, Liu D*. (2012). Suppression of Rap1 impairs cardiac myofibril and conduction system in zebrafish. PLoS ONE, 7(11): e50960. Doi:10.1371/journal.pone.0050960.
12. Liu M, Liu P, Zhang L, Gao G, Zhang W, Zhu Z, Liu D*, Fan Q*. (2011). mir-35 is involved in control of intestine cell G1/S transition and germ cell proliferation in C. elegans. Cell Res, 21(11):1605-18.
13. Choy SW, Cheng CW, Lee ST, Li VWT, Hui MNY, Hui C, Liu D, Cheng SH. (2010). A cascade of irx1a and irx2a controls shh expression during retinogenesis. Dev Dyn, 239:3204-14.
14. Yan Y-L, Willoughby J, Liu D, Crump JG, Wilson C, Miller CT, Singer A, Kimmel C, Westerfield M, Postlethwait JH. (2005). A pair of Sox: distinct and overlapping functions of zebrafish Sox9 co-orthologs in craniofacial and pectoral fin development. Development, 132: 1069-83.
15. Hans S, Liu D, Westerfield M. (2004). Pax8 and Pax2a function synergistically in otic specification, downstream of the Foxi1 and Dlx3b transcription factors. Development, 131: 5091-02.
16. Liu D, Chu H, Maves L, Yan YL, Morcos PA, Postlethwait JH, Westerfield M. (2003). Fgf3 and Fgf8 dependent and independent transcription factors are required for otic placode specification. Development, 130: 2213-24.
17. Yan Y-L, Miller CT, Nissen R, Singer A, Liu D, Kirn A, Draper B, Willoughby J, Morcos PA, Amsterdam A, Chung B, Westerfield M, Haffter P, Hopkins N, Kimmel C and Postlethwait JH. (2002). Zebrafish sox9 gene required for cartilage morphogenesis. Development, 129: 5065-79.
18. Liu D, Chandy M, Lee SK, Le Dréan Y, Ando H, Xiong F, Woon Lee J, Hew CL. (2000). A zebrafish ftz-F1 (Fushi tarazu factor 1) homologue requires multiple subdomains in the D and E regions for its transcriptional activity. J Biol Chem, 2;275(22):16758-66.
19. Liu D, Le Dréan Y, Ekker M, Xiong F, Hew CL. (1997). Teleost FTZ-F1 homolog and its splicing variant determine the expression of salmon gonadotropin II beta gene. Mol Endocrinol,11(7):877-90.
20. Wong AO, Dréan Y, Liu D, Hu Z, Xiong F, Hew CL. (1996). Induction of Chinook Salmon Growth Hormone Promoter Activity by the Adenosine 3′,5′-Monophosphate (cAMP)-Dependent Pathway Involves Two cAMP-Response Elements with the CGTCA Motif and the Pituitary-Specific Transcription Factor Pit-1. Endocrinology, 137(5):1775-84.
21. Dréan Y, Liu D, Wong AO, Xiong F, Hew CL. (1996). Steroidogenic factor 1 and estradiol receptor act in synergism to regulate the expression of the salmon gonadotropin II beta subunit gene. Mol Endocrinol,10(3):217-29.
22. Liu D, Xiong F, Hew CL. (1995) Functional analysis of estrogen-responsive elements in Chinook salmon (Oncorhynchus tschawytscha) gonadotropin II beta subunit gene. Endocrinology, 136(8):3486-93.
23. Xiong F, Liu D, Dréan Y, Elsholtz HP, Hew CL. (1994). Differential recruitment of steroid hormone response elements may dictate the expression of the pituitary gonadotropin II beta subunit gene during salmon maturation. Mol Endocrinol, 8(6):782-93.
24. Xiong F, Liu D, Dréan Y, Hew CL. (1994). The Chinook salmon gonadotropin II beta subunit gene contains a strong minimal promoter with a proximal negative element. Mol Endocrinol, 8(6):771-81.
25. Xie Y, Liu D, Zou J, Li G, Zhu Z. (1993). Gene transfer via electroporation in fish. Aquaculture, 111(1-4):207-13.
課程
教授過的課程包括：本科生《發(fā)育生物學(xué)》，研究生《細(xì)胞死亡與衰老》和《脊椎動物形態(tài)發(fā)生》，研究生專題《神經(jīng)發(fā)育與再生》。2017秋季開講《動物形態(tài)發(fā)生學(xué)》。
聯(lián)系方式
深圳市南山區(qū)西麗學(xué)苑大道1088號南方科技大學(xué)第一科研樓202室
郵編：518055
電話：0755 88018487
電子郵件：[email protected]