Faculty
Self-introduction
Kaige Yan obtained his Ph.D. degree from Tsinghua University, 2016. After a four-year postdoctoral training supervised by Prof. David Barford at the MRC Laboratory of Molecular Biology in Cambridge, Dr. Yan joined the Department of Biology in SUSTech as an assistant professor in October 2020. His research has been focused on understanding the mechanisms and regulations of chromosome segregation in mitosis, and ribonucleoprotein (RNP) complex assembly and transport, by using a combination of structural and biochemical techniques with an emphasis on high-resolution electron microscopy.
Research Interests：
◆Mechanisms and regulations of chromosome segregation in mitosis
◆Ribonucleoprotein (RNP) complex assembly and transport
Professional Experience：
◆ 2020 – Present Assistant Professor in Southern University of Science and Technology of China, Shenzhen, China.
◆ 2016 – 2020 Postdoctoral scientist in MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
Group Leader: Prof. David Barford
Educational Background：
◆ 2011 – 2016 Ph.D. candidate majored in Structural Biology, Biophysics. Received Ph.D. Degree from School of Life Sciences, Tsinghua University, China.
Advisor: Prof. Ning Gao & Jianlin Lei
◆ 2007 – 2011 Majored in Biophysics. Received B.S. Degree from Department of Applied Physics and School of Physics, Nankai University, China
Selected Publication：
1. Yan, K. ?, Yang, J. ?, Zhang, Z. ?, McLaughlin, S.H., Chang, L., Fasci, D., Ehrenhofer-Murray, A.E., Heck, A.J.R. and Barford, D.. (2019) Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome. Nature, 574, 278-282. (? contributed equally to this work).
2. Yan, K. ?,Zhang, Z. ?, Yang, J., McLaughlin, S.H. and Barford, D.. (2018) Architecture of the CBF3-centromere complex of the budding yeast kinetochore. Nature structural & molecular biology, 25, 1103-1110.
3. Zhang, D. ?, Yan, K.?, Liu, G., Song, G., Luo, J., Shi, Y., Cheng, E., Wu, S., Jiang, T., Lou, J., Gao, N. and Qin, Y.. (2016) EF4 disengages the peptidyl-tRNA CCA end and facilitates back-translocation on the 70S ribosome. Nature structural & molecular biology, 23, 125-131.
4. Zhang, D. ?, Yan, K.?, Zhang, Y. ?, Liu, G., Cao, X., Song, G., Xie, Q., Gao, N. and Qin, Y.. (2015) New insights into the enzymatic role of EF-G in ribosome recycling. Nucleic acids research, 43, 10525-10533.
5. Zhang, X. ?, Yan, K.?, Zhang, Y., Li, N., Ma, C., Li, Z., Feng, B., Liu, J., Sun, Y., Xu, Y., Lei, J. and Gao, N.. (2014) Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly. Nucleic acids research, 42, 13430-13439.
6. Wang, W., Li, W., Ge, X., Yan, K., Mandava, C.S., Sanyal, S. and Gao, N.. (2020) Loss of a single methylation in 23S rRNA delays 50S assembly at multiple late stages and impairs translation initiation and elongation. Proc Natl Acad Sci U S A, 117, 15609-15619.
7. Ma, C., Wu, S., Li, N., Chen, Y., Yan, K., Li, Z., Zheng, L., Lei, J., Woolford, J.L.J. and Gao, N.. (2017) Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1. Nature structural & molecular biology, 24, 214-220.
8. Wu, S., Tutuncuoglu, B., Yan, K., Brown, H., Zhang, Y., Tan, D., Gamalinda, M., Yuan, Y., Li, Z., Jakovljevic, J., Ma, C., Lei, J., Dong, M., Woolford, J.L.J. and Gao, N.. (2016) Diverse roles of assembly factors revealed by structures of late nuclear pre-60S ribosomes. Nature, 534, 133-137.
9. Gu, J., Wu, M., Guo, R., Yan, K., Lei, J., Gao, N. and Yang, M.. (2016) The architecture of the mammalian respirasome. Nature, 537, 639-643.
10. Ma, C., Yan, K., Tan, D., Li, N., Zhang, Y., Yuan, Y., Li, Z., Dong, M., Lei, J. and Gao, N.. (2016) Structural dynamics of the yeast Shwachman-Diamond syndrome protein (Sdo1) on the ribosome and its implication in the 60S subunit maturation.Protein & cell. 7,187-200.
11. Zhang, J., Pan, X., Yan, K., Sun, S., Gao, N. and Sui, S.F.. (2015) Mechanisms of ribosome stalling by SecM at multiple elongation steps. eLife, 4.
12. Yang, Z., Guo, Q., Goto, S., Chen, Y., Li, N., Yan, K., Zhang, Y., Muto, A., Deng, H., Himeno, H., Lei, J. and Gao, N.. (2014) Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence. Protein & cell, 5, 394-407.